Micropipetting Exercise
Learning Outcome #3
- Prepares reagents, standards, and solutions to industry standards for use in the clinical lab, utilizing common lab equipment according to established protocols.
Supplies
- Kimwipes
- Distilled water
- Micro pipette
Instructions
Read the list of required equipment and supplies and the procedural steps. Follow the procedural steps in the exact order.
Have instructor check off steps.
| Procedural Step | Instructor |
|---|---|
| Put on gloves and eye protection | |
| Assemble equipment and supplies | |
| Place an empty weigh boat on the clean balance | |
| Set the balance to zero | |
| Add 100uL of distilled H20 to the weigh boat and record the weight | |
| Repeat steps 1-3 for a total of 3 readings | |
| Calculate the average of the 3 measurements | |
| Multiply the average by 1000 to calculate the number of uL the pipette has delivered |
| Setting on Micropipette | Weight of Water |
|---|---|
| 100 uL | |
| 100 uL | |
| 100 uL | |
| Average weight | |
| Average weight x 1000 |
| Micro Pipette Practice | Initials |
|---|---|
| Correct semi-automatic pipette adjustment for volume | |
| Correct “stops” when filling and delivering semi-automatic pipette | |
| Tip against receiving vessel when draining |