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About this Manual
1. Transfusion Medicine Lab Guidelines
2. Laboratory Evaluation Rubric
3. Professional Conduct Rubric
4. Nova Scotia Community College Medical Laboratory Technology Program
5. Transfusion Medicine Student Cupboard Supplies
6. Cell Washers Information and Troubleshooting
7. Cell Washer Maintenance Charts
8. Inverted Microscope Parts and Information
9. Inverted Microscope Maintenance Charts
10. Quality Control Worksheet
11. Dry Bath Maintenance Chart
12. Specimen Acceptance/Separation/Labeling/Storage
13. Patient History Check
14. Patient Records
15. Tube Labeling
16. Board / Rack Set-Up
17. Reading, Grading, and Recording Reactions
18. Preparation of Red Cell Suspensions
19. Cell Washing: Manual Method
20. Cell Washing: Automated Method
21. Tube Tests for ABO Typing
22. ABO Worksheet
23. Resolving ABO Anomalies
24. Saline Replacement for Rouleaux
25. Saliva Testing for ABH Substances
26. Tube Tests for Rh Typing of Red Cells
27. Testing for Weak D
28. ABO and Rh Worksheet
29. Exercise on ABO/Rh groupings
30. ABO Discrepancies/Anomalies Exercise
31. Phenotyping -“Direct” Method
32. Phenotyping - “Indirect” Method
33. Manufacturer’s Instructions for Phenotyping Red Cells
34. Phenotyping Worksheet
35. Prediction of Rh Genotypes from Phenotypes
36. Direct Antiglobulin Test (DAT)
37. Antibody Detection and Identification Flowchart
38. Group/Screen (ABS) and BB Hold Procedure
39. Antibody Screening by Saline IAT (SIAT)
40. Antibody Screening by LISS IAT
41. Antibody Identification - LISS
42. Antibody Screening (ABS) by Gel Method (G-IAT)
43. Antibody Identification (ABID) – Gel Method (G-IAT)
44. Student Exercise on unexpected antibodies
45. Antibody Identification (Matching or Exclusion)
46. Selected Panels (cells)
47. “Oscar F” Case Study Exercise
48. Crossmatching Flow Chart
49. Immediate-spin Crossmatch
50. LISS Antihuman Globulin (AHG) Crossmatch
51. Crossmatch GEL-MTS Method
52. Antibody Identification, Exclusions and Confirmations…
53. ABS by Micro Typing Systems (MTS)-Extended
54. ABID by Micro Typing Systems (MTS)- extended
55. MTS Maintenance Chart
56. Problem Solving Exercise on Antibody Identification
57. Pre-warming Technique
58. GammaZyme-B Bromelin Solution™
59. Fetal Maternal Testing Flow Chart
60. Prenatal Screening
61. Rh Immune Globulin (RhIg)
62. A Mini Panel for Passive Anti-D (RhIg)
63. Cord Specimens
64. Fetal Bleed Screening Test
65. Kleihauer-Betke Test (Modified) (using a “Simmler” kit)
66. Postnatal and Cord lab Instructions
67. Mom and Baby Exercise
68. Antibody Titration
69. Acid Elution (ELU KITTM PLUS)
70. Elution Student Questions
71. Problem Solving Exercise on DAT’s and Elutions
72. “General” Warm-Reactive Autoantibody Adsorption Procedure
73. WAIHA Elutions using Warm Autoantibody Removal Medium (W.A.R.M.)
74. Investigation of Adverse Effects of Transfusion
75. Problem Solving Exercise on Transfusion Reactions
76. Lab Summary - Student Assessment
References
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